Tumor Associated Tissue Eosinophilia As a Potential Predictor in The Invasion Patterns of Oral Squamous Cell Carcinoma

Contributors: 1Senior Lecturer, Department of Oral Pathology & Microbiology, Bhojia Dental College and Hospital, Baddi, Himachal Pradesh, India; 2Professor, Department of Preventive Dental Science, College of Dentistry, Prince Sattam bin Abdulaziz University, Al-Kharj, Saudi Arabia; 3Professor, Department of Oral Pathology and Microbiology, Dr. D. Y. Patil Dental College and Hospital, Maheshnagar, Pimpri, Pune, Maharashtra, India; 4Associate Professor, Department of Oral Pathology and Microbiology, Dr. D. Y. Patil Dental College and Hospital, Maheshnagar, Pimpri, Pune, Maharashtra, India; 5Professor and Head, Department of Oral Pathology & Microbiology, Faculty of Dental Sciences, MS Ramaiah University of Applied Sciences, Bengaluru, Karnataka, India; 6Associate Professor, Department of Maxillofacial Surgery and Diagnostic Sciences, Division of Oral Pathology, College of Dentistry, Jazan University, Jazan, Saudi Arabia. Correspondence: Dr. Patil S. Department of Maxillofacial Surgery and Diagnostic Sciences, Division of Oral Pathology, College of Dentistry, Jazan University, Jazan, Saudi Arabia. Email: [email protected] How to cite the article: Majumdar B, Anil S, Sarode SC, Sarode GS, Rao RS, Patil S. Tumor associated tissue eosinophilia as a potential predictor in the invasion patterns of oral squamous cell carcinoma. J Int Oral Health 2016;8(11):1026-1030. Abstract: Background: Eosinophils are a rare type of granular leukocyte comprising only 1-4% of the total white blood count. Their presence within a wide variety of human cancers raises queries about their role. The pattern of invasion (POI) in oral squamous cell carcinoma (OSCC) has been recognized as an adverse prognostic feature. Different theories have been proposed linking tumor-associated tissue eosinophilia (TATE) and its role in tumor progression. Hence, this study aims to assess the role of TATE in the invasion pattern of OSCC. Materials and Methods: It is a cross-sectional study of a retrospectively collected 70 cases of OSCC, drafted from the institutional archive (2011-2014). The archival paraffin-embedded tissues were retrieved and subjected to sectioning and staining with hematoxylin and eosin and carbol chromotrope (Lendrum, 1944). The TATE was graded based on modified classical counting method (Alkhabuli and High, 2006), and the POI was evaluated based on Bryne’s invasive tumor front grading system (1989). Chi-square test (P < 0.05) was employed to assess the relationship between the selected parameters. Results: Statistically significant association was found between TATE count at the invasive front and POI (P < 0.001), histological grading (P < 0.002), and the TATE count at the noninvasive front (intraand stroma subadjacent to surface epithelium) (P < 0.010). The patient demographics and nodal metastases correlation with TATE count resulted in insignificant statistical association. Conclusion: Thus, the positive correlation between TATE and POI suggests that the eosinophils might play a role in tumorcytotoxicity and affect tumor growth.